Contents: Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)
Catalog Number: 53-0031
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 145-2C11
Isotype: Armenian Hamster IgG
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Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 μg of Alexa Fluor® 488 anti-mouse CD3e (145-2C11) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 145-2C11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Leo, O., M. Foo, et al. (1987). "Identification of a monoclonal antibody specific for a murine T3 polypeptide."
Proc Natl Acad Sci U S A 84(5): 1374-8.
Samelson, L. E., J. J. O'Shea, et al. (1987). "T cell antigen receptor phosphorylation induced by an anti-receptor antibody."
J Immunol 139(8): 2708-14.
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Cat.
53-4888 Alexa Fluor® 488 Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart