Product Information

Contents: Alexa Fluor® 488 anti-human CD1a
Catalog Number: 53-0019
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI149
Isotype: Mouse IgG1, κ
HLDA No.: V 5T CD01.01

Staining of MOLT-4 cells with Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (open histogram) or Alexa Fluor® 488 anti-human CD1a (HI149) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0019	Affinity Purified anti-human CD1a	N/A	N/A	FC IHC
13-0019	Biotin anti-human CD1a	N/A	N/A	FC
11-0019	FITC anti-human CD1a	488	518	FC
12-0019	PE anti-human CD1a	488	575	FC
51-0019	Alexa Fluor® 647 anti-human CD1a	633	668	FC
53-0019	Alexa Fluor® 488 anti-human CD1a	488	519	FC
57-0019	Pacific Blue® anti-human CD1a	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI149 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.