Product Information

Contents: Alexa Fluor® 647 anti-human HLA- MHC Class I free chain without B2 microglobulin
Catalog Number: 51-9958
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A4
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells either unactivated (left) or 3-day PHA-activated (right) with Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (open histogram) or Alexa Fluor® 647 anti-human HLA- MHC Class I free chain without B2 m (A4) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9958	Affinity Purified anti-human HLA- MHC Class I free chain without B2 microglobulin	N/A	N/A	FC IHC(Paraffin) IP
51-9958	Alexa Fluor® 647 anti-human HLA- MHC Class I free chain without B2 microglobulin	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of β2 microglobulin. There are three class I α-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with β2 microglobulin (β2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This A4 antibody has been pre-titrated and tested by flow cytometric analysis of 3-day PHA-activated PBMC. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Lopez de Castro JA, Barbosa JA, Krangel MS, Biro PA, Strominger JL. Structural analysis of the functional sites of class I HLA antigens. Immunol Rev. 1985 Jul;85:149-68.

Related Products

Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.