Product Information

Contents: Alexa Fluor® 647 anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)
Catalog Number: 51-9024
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: T2.5
Isotype: Mouse IgG1

Staining of C57Bl/6 splenocytes with FITC anti-mouse/human CD11b (M1/70) (cat. 11-0112) and staining buffer (autofluorescence) (left) or 0.25 μg of Alexa Fluor® 647 anti-mouse/human TLR2 (T2.5) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9024	Affinity Purified anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FA FC IHC IP
16-9024	Functional Grade* Purified anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FA FC
13-9024	Biotin anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
12-9024	Phycoerythrin (PE) anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
25-9024	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	760	FC
51-9024	Alexa Fluor® 647 anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	668	FC
53-9024	Alexa Fluor® 488 anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	519	FC
57-9024	Pacific Blue® anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The T2.5 monoclonal antibody reacts with mouse and human Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens. T2.5 is an antagonistic antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This T2.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This T2.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Meng G, Rutz M, Schiemann M, Metzger J, Grabiec A, Schwandner R, Luppa PB, Ebel F, Busch DH, Bauer S, Wagner H, Kirschning CJ. 2004. Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes. J Clin Invest. 2004. 113:1473-81.

Sutmuller RP, den Brok MH, Kramer M, Bennink EJ, Toonen LW, Kullberg BJ, Joosten LA, Akira S, Netea MG, Adema GJ. Toll-like receptor 2 controls expansion and function of regulatory T cells. J Clin Invest. 2006 Feb;116(2):485-94 (T2.5, FC, PubMed)

Zhang C, Wang SH, Lasbury ME, Tschang D, Liao CP, Durant PJ, Lee CH. Toll-like receptor 2 mediates alveolar macrophage response to Pneumocystis murina. Infect Immun. 2006 Mar;74(3):1857-64 (T2.5, FA, PubMed).

Leemans JC, Stokman G, et al. 2005. Renal-associated TLR2 mediates ischemia/reperfusion injury in the kidney. J Clin Invest. 2005 Oct;115(10):2894-903. (IHC frozen and IP, PubMed)

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