Product Information

Contents: Alexa Fluor® 647 anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)
Catalog Number: 51-9021
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6C2
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 0.06 μg of Alexa Fluor® 647 Rat IgG2b Isotype Control (cat. 51-4031) (left) or 0.06 μg of Alexa Fluor® 647 anti-mouse TLR2 (6C2) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9021	Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F IHC(Paraffin) IP
16-9021	Functional Grade* Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
13-9021	Biotin anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F
11-9021	FITC anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9021	PE anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
17-9021	DISCONTINUED - Please see newly released Alexa Fluor® 647 6C2 as an alternate Allophycocyanin (APC) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	660	FC
51-9021	Alexa Fluor® 647 anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This 6C2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gibson FC 3rd, Hong C, Chou HH, Yumoto H, Chen J, Lien E, Wong J, Genco CA. 2004. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation. 109(22):2801-6.
Paterson HM, Murphy TJ, Purcell EJ, Shelley O, Kriynovich SJ, Lien E, Mannick JA, Lederer JA. Injury Primes the Innate Immune System for Enhanced Toll-Like Receptor Reactivity. J Immunol. 2003 Aug 1;171(3):1473-1483.
Nilsen NJ, Nonstad U, Khan N, Knetter CF, Akira S, Sundan A, Espevik T, Lien E. 2004. Lipopolysaccharide and double-stranded RNA upregulate toll-like receptor 2 independently of myeloid differentiation factor 88. J Biol Chem. 279(38):39727-35.

Related Products

Cat. 51-4031    Alexa Fluor® 647 Rat IgG2b Isotype Control
Cat. 51-9024    Alexa Fluor® 647 anti-mouse/human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone T2.5)
Cat. 51-9922    Alexa Fluor® 647 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2) (clone TL2.1)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.