Product Information

Contents: Alexa Fluor® 647 anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)
Catalog Number: 51-9011
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioTR23 (TR23)
Isotype: Rat IgG2a, κ

Staining of mouse TLR1-transfected Baf/3 cells with 0.5 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (open histogram) or 0.5 μg of Alexa Fluor® 647 anti-mouse TLR1 (eBioTR23) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9011	Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC IP
13-9011	Biotin anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC
12-9011	Phycoerythrin (PE) anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	488	575	FC
51-9011	Alexa Fluor® 647 anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioTR23 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281). TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function. Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis. TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioTR23 (TR23) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioTR23 (TR23) antibody has been tested by flow cytometric analysis of mouse TLR1-transfected Baf/3 cells and mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Alexopoulou L, Thomas V, Schnare M, Lobet Y, Anguita J, Schoen RT, Medzhitov R, Fikrig E, Flavell RA. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 2002 Aug;8(8):878-84.

Wyllie DH, Kiss-Toth E, Visintin A, Smith SC, Boussouf S, Segal DM, Duff GW, Dower SK. Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol. 2000 Dec 15;165(12):7125-32.

Takeuchi O, Sato S, Horiuchi T, Hoshino K, Takeda K, Dong Z, Modlin RL, Akira S. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J Immunol. 2002 Jul 1;169(1):10-4.

Related Products

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Cat. 14-9012  Affinity Purified anti-mouse Toll-like receptor 1 (CD281, TLR1, TLR-1) (clone eBioLD5)
Cat. 12-9911  PE anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1) (clone GD2.F4)
Cat. 14-9911  Affinity Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1) (clone GD2.F4)
Cat. 16-9911  Functional Grade Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1) (clone GD2.F4)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.