Contents: Alexa FluorŽ 647 anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)
Catalog Number: 51-8813
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-480 (MC-480)
Isotype: Mouse IgM
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Staining of F9 cells with staining buffer (autofluorescence) (blue histogram) or 0.06 μg of Alexa FluorŽ 647 anti-mouse/human SSEA-1 (MC-480) (purple histogram). Total viable cells were used for analysis.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of F9 mouse cell line. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Anjos-Afonso F, Bonnet D. Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment.
Blood. 2007 Feb 1;109(3):1298-306. (
PubMed)
Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens.
Methods Mol Biol. 2006;325:207-24. (
PubMed)
Solter D, Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9. (
PubMed)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart