Product Information

Contents: Alexa Fluor® 647 anti-human GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)
Catalog Number: 51-7337
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BVD2-21C11
Isotype: Rat IgG2a

Normal human peripheral blood mononuclear cells were restimulated with PMA and Ionomycin in the presence of monensin for 5 hours and then stained intracellularly with FITC anti-human CD4 (RPA-T4) (cat. 11-0049) and 0.03 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or 20µl of Alexa Fluor® 647 anti-human GM-CSF (BVD2-21) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-7337	Alexa Fluor® 647 anti-human GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	633	668	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The BVD2-21C11 monoclonal antibody reacts with human Granulocyte/Monocyte-Colony Stimulating Factor (GM-CSF). Human GM-CSF is a 127 amino acid, differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF-α, IL-1, IL-2 and IFN-γ. GM-CSF is found associated with the extracellular matrix and in membrane-bound formats as well. It stimulates proliferation, activation and differentiation of macrophages, granulocytes and their progenitors.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BVD2-21C11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This BVD2-21C11 antibody has been pre-titrated and tested on peripheral blood mononuclear cells reactivated with PMA and Ionomycin in the presence of monensin. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume.

References

Bacchetta et al (1990). Host reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol 144(3):902-908.

Kita et al (1991). Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils. J Exp Med 174(3):745-748.

Prussin et al (1995). Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods 188(1): 117-128.

Kitamura et al (1999). Idiopathic Pulmonary Alveolar Proteinosis as an Autoimmune Disease with Neutralizing Antibody against Granulocyte/Macrophage Colony-Stimulating Factor. J Exp Med 190 (6): 875.

Related Products

Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.