Product Information

Contents: Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70
Catalog Number: 51-7235
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioHP40 (HP40, HP-40)
Isotype: Mouse IgG1, κ

Intracellular staining of IFN-γ-primed, LPS-stimulated human myeloid derived dendritic cells.
Left plot: Surface staining with FITC anti-human CD11c (3.9) (cat. 11-0116) followed by fixation, permeabilization, and intracellular staining with Alexa Fluor® 647 anti-human IL-12 (eBioHP40).
Right Plot: Intracellular co-staining of Alexa Fluor® 647 eBioHP40 and PE anti-human IL-12, clone C8.6 (cat. 12-7129).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7235	Phycoerythrin (PE) anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	488	575	IC Flow
51-7235	Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	633	668	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioHP40 (HP40, HP-40) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining of stimulated DC followed by flow cytometric analysis. This can be used at 20 μl (0.015 μg) per million cells in a 100 μl total staining volume.

References

Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.

Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.

Related Products

Cat. 51-4714    Alexa Fluor® 647 Mouse IgG1, K Isotype Control
Cat. 51-7123    Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23), p40 (clone C17.8)
Cat. 51-7129    Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 (clone C8.6)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.