Product Information

Contents: Alexa Fluor® 647 anti-human IL-21 (Interleukin-21, IL21)
Catalog Number: 51-7219
Sizes: 25 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio3A3-N2 (3A3-N2)
Isotype: Mouse IgG1

Intracellular staining of unstimulated (left) or PMA/ionomycin/monensin (5 hour) stimulated (right) human PBMCs with FITC anti-human CD4 (RPA-T4) (cat. 11-0049) and Alexa Fluor® 647 anti-human IL-21 (3A3-N2). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7219	Phycoerythrin (PE) anti-human IL-21 (Interleukin-21, IL21)	488	575	IC Flow
51-7219	Alexa Fluor® 647 anti-human IL-21 (Interleukin-21, IL21)	633	668	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by flow cytometry analysis of stimulated normal human PBMCs. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

Staining for IL-21 has shown donor variability. Stimulation times may vary depending on the donor and should be optimized. Please contact Technical support at tech@ebioscience.com for additional information.

References

Parrish-Novak J, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000 Nov 2;408(6808):57-63. PubMed.

Parrish-Novak J, Foster DC, Holly RD, Clegg CH.Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 2002 Nov;72(5):856-63. PubMed.

Related Products

Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.