Product Information

Contents: Alexa Fluor® 647 anti-mouse IL-13 (Interleukin-13, IL13)
Catalog Number: 51-7133
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio13A
Isotype: Rat IgG1, κ

BALB/c splenocytes were cultured for 3 days with plate-bound anti-mouse CD3e (clone 145-2C11, cat. 16-0031), soluble anti-mouse CD28 (clone 37.51, cat. 16-0281), recombinant mouse IL-2 (cat. 14-8021), and recombinant mouse IL-4 (cat. 14-8041). Cells were then harvested and restimulated with PMA/Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with FITC anti-mouse CD4 (clone RM4-5, cat. 11-0042), fixed and permeabilized, then stained with 0.06 μg Alexa Fluor 647 eBio13A (left). The ligand blocking control demonstrates that pre-blocking the Alexa Fluor 647 conjugated eBio13A antibody with 1μg of recombinant mouse IL-13 (cat. 14-8131) prior to staining abrogates specific mouse IL-13 staining (right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7133	Phycoerythrin (PE) anti-mouse IL-13 (Interleukin-13, IL13)	488	575	IC Flow
51-7133	Alexa Fluor® 647 anti-mouse IL-13 (Interleukin-13, IL13)	633	668	IC Flow
14-7133	Affinity Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA cap
16-7133	Functional Grade* Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISPOT cap
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.

Applications Tested

This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miyahara, N., et al. 2004. Contribution of antigen-primed CD8+T cells to the develompent of airwary hyperresponsiveness and inflammation is associated with IL-13. J. Immunol. 172: 2539-2558.

McKenzie AN, Li X, Largaespada DA, Sato A, Kaneda A, Zurawski SM, Doyle EL, Milatovich A, Francke U, Copeland NG, et al. Structural comparison and chromosomal localization of the human and mouse IL-13 genes. J Immunol. 1993 Jun 15;150(12):5436-44.

Doherty TM, Kastelein R, Menon S, Andrade S, Coffman RL. Modulation of murine macrophage function by IL-13. J Immunol. 1993 Dec 15;151(12):7151-60.

Terabe M, Matsui S, Noben-Trauth N, Chen H, Watson C, Donaldson DD, Carbone DP, Paul WE, Berzofsky JA. NKT cell-mediated repression of tumor immunosurveillance by IL-13 and the IL-4R-STAT6 pathway. Nat Immunol. 2000 Dec;1(6):515-20.

Grunig G, Warnock M, Wakil AE, Venkayya R, Brombacher F, Rennick DM, Sheppard D, Mohrs M, Donaldson DD, Locksley RM, Corry DB. Requirement for IL-13 independently of IL-4 in experimental asthma. Science. 1998 Dec 18;282(5397):2261-3.

Related Products

Cat. 51-4301    Alexa Fluor® 647 Rat IgG1 Isotype Control
Cat. 53-7041    Alexa Fluor® 488 anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 53-7101    Alexa Fluor® 488 anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-16E3)
Cat. 88-7134    Mouse IL-13 (Interleukin-13, IL13) ELISA Ready-SET-Go!
Cat. 88-7825    Mouse IL-5 (Interleukin-5, IL5) ELISPOT Ready-Set-Go!
Cat. 34-8131    Carrier-Free Recombinant Mouse IL-13 (Interleukin-13, IL13)

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