Product Information

Contents: Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40
Catalog Number: 51-7123
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C17.8
Isotype: Rat IgG2a, κ

Intracellular IL-12 p40 staining. C57BL/6 thioglycolate-elicted peritoneal exudate cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. The cells fixed, permeabilized, and stained with Alexa Fluor® 647 anti-mouse IL-12 (colored histogram). The open histogram represents autofluorescence. Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7123	PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	488	575	IC Flow
13-7123	Biotin anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	ELISA det
16-7123	Functional Grade* Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	IC Flow IP NU WB
45-7123	PerCP-Cy5.5 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40	488	690	IC Flow
51-7123	Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23), p40	633	668	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This C17.8 antibody has been reported for use in ELISA, neutralization, and intracellular staining with flow cytometric analysis.

Applications Tested

This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB.
Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T.
IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.

Related Products

Cat. 51-4321    Alexa Fluor® 647 Rat IgG2a Isotype Control
Cat. 14-7125    Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C15.6)
Cat. 16-7125    Functional Grade Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C15.6)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.