Product Information

Contents: Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10)
Catalog Number: 51-7108
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JES3-9D7
Isotype: Rat IgG1, κ

CD4+ T cells were cultured for 4 days under Th2 polarizing conditions. Cells were then reactivated with PMA/Ionomycin (right) or unstimulated (left) in the presence of monensin for 5 hrs. Cells were harvested, stained with anti-CD3 (11-0037), fixed, permeabilized, and stained with Alexa Fluor® 647 anti-human IL-10.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7108	PE anti-human IL-10 (Interleukin-10, IL10)	488	575	IC Flow
14-7108	Affinity Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap IHC(Paraffin)
16-7108	Functional Grade* Purified anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISPOT cap NU
51-7108	Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10)	633	668	IC Flow
53-7108	Alexa Fluor® 488 anti-human IL-10 (Interleukin-10, IL10)	488	519	IC Flow
57-7108	Pacific Blue® anti-human IL-10 (Interleukin-10, IL10)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th2-polarized CD4+ T cells. This can be used at less than or equal to 20 μl (0.125 μg) per million cells in a 100 μl total staining volume, however the titre may need to be further optimized in some experiments.

References

Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.

Chandler SW, Rassekh CH, et al. 2002. Immunohistochemical localization of interleukin-10 in human oral and pharyngeal carcinomas. Laryngoscope. 112(5):808-15. (IHC paraffin, PubMed)

Sendide K, Deghmane AE, et al. 2005. Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S. J Immunol. 175(8):5324-32. (FA, PubMed)

Related Products

Cat. 51-4301 Alexa Fluor® 647 Rat IgG1 Isotype Control
Cat. 51-7101 Alexa Fluor® 647 anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-16E3)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.