Product Information

Contents: Alexa Fluor® 647 anti-mouse IL-10 (Interleukin-10)
Catalog Number: 51-7101
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JES5-16E3
Isotype: Rat IgG2b, κ

Staining with Alexa Fluor® 647 anti-mouse IL-10. Mouse splenocytes were stimulated with ConA for 2 days, rested in IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. Cells were harvested, stained with FITC anti-mouse CD4, fixed, permeabilized, and stained intracellularly with Alexa Fluor® 647 anti-mouse IL-10 (Right), in comparison with isotype control (Left). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7101	FITC anti-mouse IL-10 (Interleukin-10, IL10)	488	518	IC Flow
12-7101	PE anti-mouse IL-10 (Interleukin-10, IL10)	488	575	IC Flow
14-7101	Affinity Purified anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap
16-7101	Functional Grade* Purified anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap NU
17-7101	APC anti-mouse IL-10 (Interleukin-10, IL10)	633	660	IC Flow
36-7101	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	IVCCA
45-7101	PerCP-Cy5.5 anti-mouse IL-10 (Interleukin-10)	488	690	IC Flow
51-7101	Alexa Fluor® 647 anti-mouse IL-10 (Interleukin-10, IL10)	633	668	IC Flow
53-7101	Alexa Fluor® 488 anti-mouse IL-10 (Interleukin-10, IL10)	488	519	IC Flow
56-7101	Alexa Fluor® 700 anti-mouse IL-10 (Interleukin-10)	633	723	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.

Applications Tested

The Alexa Fluor® 647 - conjugated JES5-16E3 antibody has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.125 µg antibody per million cells in 100 ul volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 1662: 201-14.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

Cat. 51-4031    Alexa Fluor® 647 Rat IgG2b Isotype Control
Cat. 13-7102    Biotin anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-2A5)
Cat. 16-7102    Functional Grade Purified anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-2A5)
Cat. 88-7904    Mouse IL-10 (Interleukin-10, IL10) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

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