Product Information

Contents: Alexa Fluor® 647 anti-human IL-9 (Interleukin-9)
Catalog Number: 51-7097
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MH9A4
Isotype: Mouse IgG2b

Staining of Th2-polarized, PMA and Ionomycin-restimulated peripheral blood mononuclear cells with FITC anti-human CD4 (RPA-T4) (cat. 11-0049) and 0.06 μg of Mouse IgG2b Iso Cntrl Alexa Fluor® 647(cat. 51-4732) (left) or 0.06 μg of anti-human IL-9 Alexa Fluor® 647 (MH9A4) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-7097	Alexa Fluor® 647 anti-human IL-9 (Interleukin-9)	633	668	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody MH9A4 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF-β or differentiated directly from naïve CD4+ T cells with TGF-β and IL-4.

IL-9 is a member of the common cytokine receptor γ chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9A4 antibody is not blocked by the MH9D1 antibody, suggesting that the two antibodies bind to unique epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MH9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This MH9A4 antibody has been pre-titrated and tested on PMA and Ionomycin-restimulated Th2-polarized CD4+ PBMC. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.

References

Jenmalm MC, Van Snick J, Cormont F, Salman B. Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children. Clin Exp Allergy. 2001 Oct;31(10):1528-35.

Renauld JC, Houssiau F, Louahed J, Vink A, Van Snick J, Uyttenhove C. Interleukin-9. Adv Immunol. 1993;54:79-97.

Related Products

Cat. 51-4732  Alexa Fluor® 647 Mouse IgG2b Isotype Control
Cat. 12-7098  Phycoerythrin (PE) anti-human IL-9 (Interleukin-9) (clone MH9D1)
Cat. 88-7958  Human IL-9 ELISA Ready-SET-Go!
Cat. 14-8958  Recombinant human IL-9

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.