Product Information

Contents: Alexa Fluor® 647 anti-human IL-4 (Interleukin-4, IL4)
Catalog Number: 51-7049
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 8D4-8
Isotype: Mouse IgG1, κ

Intracellular staining of normal human peripheral blood mononuclear cells unstimulated (left) and stimulated with PMA and ionomycin in the presence of brefeldin A for 5 hours (right) with FITC anti-human CD4 (OKT4) (cat. 11-0048) and Alexa Fluor® 647 anti-human IL-4 (8D4-8). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7049	Phycoerythrin (PE) anti-human IL-4 (Interleukin-4, IL4)	488	575	IC Flow
14-7049	Affinity Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA cap ELISPOT cap
17-7049	Allophycocyanin (APC) anti-human IL-4 (Interleukin-4, IL4)	633	660	IC Flow
25-7049	Phycoerythrin-Cy7 (PE-Cy7) anti-human IL-4 (Interleukin-4, IL4)	488	760	IC Flow
51-7049	Alexa Fluor® 647 anti-human IL-4 (Interleukin-4, IL4)	633	668	IC Flow
53-7049	Alexa Fluor® 488 anti-human IL-4 (Interleukin-4, IL4)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This 8D4-8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Bird, C., et al. 1991. Development of immunoassays for human interleukin-3 and interleukin-4, some of which discriminate between different recombinant cDNA-derived molecules. Cytokine. 3: 562-567.

Related Products

Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control
Cat. 51-7041 Alexa Fluor® 647 anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.