Product Information

Contents: Alexa Fluor® 647 anti-mouse IL-4 (Interleukin-4)
Catalog Number: 51-7041
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 11B11
Isotype: Rat IgG1, κ

Intracellular IL-4 staining. C57Bl/6 splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. Autofluorescence is indicated by the open histogram; staining by 0.125 μg of Alexa Fluor® 647 anti-mouse IL-4 is demonstrated by the colored histogram.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7041	Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA cap ELISPOT cap
16-7041	Functional Grade* Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	NU
12-7041	PE anti-mouse IL-4 (Interleukin-4, IL4)	488	575	IC Flow
17-7041	APC anti-mouse IL-4 (Interleukin-4, IL4)	633	660	IC Flow
51-7041	Alexa Fluor® 647 anti-mouse IL-4 (Interleukin-4, IL4)	633	668	IC Flow
53-7041	Alexa Fluor® 488 anti-mouse IL-4 (Interleukin-4, IL4)	488	519	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 11B11 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-4 bioactivity, IHC, and intracellular staining for flow cytometric analysis.

Applications Tested

This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ohara, J. and W. E. Paul. 1985. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 315: 333-336.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

Cat. 51-4301  Alexa Fluor® 647 Rat IgG1 Isotype Control
Cat. 11-7042  FITC anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD6-24G2)
Cat. 13-7042  Biotin anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD6-24G2)
Cat. 14-7042  Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD6-24G2)
Cat. 25-7042  Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD6-24G2)
Cat. 36-7043  In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD4-1D11)
Cat. 88-7944  Mouse IL-4 (Interleukin-4, IL4) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.