Product Information

Contents: Alexa Fluor® 647 anti-mouse IgD
Catalog Number: 51-5993
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 11-26c (11-26)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.5 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (open histogram) or Alexa Fluor® 647 anti-mouse IgD (11-26) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5993	FITC anti-mouse IgD	488	518	FC
12-5993	PE anti-mouse IgD	488	575	FC
51-5993	Alexa Fluor® 647 anti-mouse IgD	633	668	FC
14-5993	Affinity Purified anti-mouse IgD	N/A	N/A	FC
13-5993	Biotin anti-mouse IgD	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 11-26c monoclonal antibody reacts with the δ heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nitschke L, Kosco MH, Koehler G, and Lamers MC. 1993. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl. Acad. Sci. USA. 90:1887-1891.
Ishihara K, Wood Jr. WJ, Wall R, Sakaguchi N, Michnoff C, Tucker PW, and Kincade PW.1993. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J. Immunol. 150: 2253 - 2262.

Related Products

Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.