Contents: Alexa FluorŽ 647 anti-mouse IgD
Catalog Number: 51-5993
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 11-26c (11-26)
Isotype: Rat IgG2a, κ
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Staining of C57Bl/6 splenocytes with 0.5 μg of Alexa FluorŽ 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (open histogram) or Alexa FluorŽ 647 anti-mouse IgD (11-26) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The 11-26c monoclonal antibody reacts with the δ heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Nitschke L, Kosco MH, Koehler G, and Lamers MC. 1993. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl. Acad. Sci. USA. 90:1887-1891.
Ishihara K, Wood Jr. WJ, Wall R, Sakaguchi N, Michnoff C, Tucker PW, and Kincade PW.1993. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J. Immunol. 150: 2253 - 2262.
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Cat.
51-4321 Alexa FluorŽ 647 Rat IgG2a Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart