Product Information

Contents: Alexa Fluor® 647 anti-mouse BTLA (CD272, B and T lymphocyte attenuator)
Catalog Number: 51-5956
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 8F4
Isotype: Mouse IgG1, κ

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or 0.06 μg of Alexa Fluor® 647 anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5956	Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator)	488	575	FC
51-5956	Alexa Fluor® 647 anti-mouse BTLA (CD272, B and T lymphocyte attenuator)	633	668	FC
14-5956	Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking	N/A	N/A	FC
13-5956	Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining. However, in staining of splenocytes and bone marrow cells, clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 8F4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 8F4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM. 2003. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 4 (7): 670-9.

Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. 2005. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. (6)1: 90-8.

Hurchla MA, Sedy JR, Gavrielli M, Drake CG, Murphy TL, Murphy KM. 2005. B and T Lymphocyte Attenuator exhibits structural expression polymorphisms and is highly induced in CD4+ T cells. The Journal of Immunology. 174: 3377-3385.

Related Products

Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.