Product Information

Contents: Alexa Fluor® 647 anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)
Catalog Number: 51-5943
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio7H22-E16
Isotype: Rat IgG1, κ

Staining of BALB/c splenocytes with PE anti-mouse CD19 (1D3) (cat. 12-0193) and 0.5 μg of Alexa Fluor® 647 Rat IgG1 Iso Cntrl (cat. 51-4301) (left) or 0.5 μg of Alexa Fluor® 647 anti-mouse BAFF R (eBio7H22-E16) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5943	Fluorescein isothiocyanate (FITC) anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	488	518	FC
51-5943	Alexa Fluor® 647 anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	633	668	FC
14-5943	Affinity Purified anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	N/A	N/A	FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio7H22.E16 reacts with mouse BAFF-Receptor, a type III transmembrane TNF receptor family member. BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice. The receptor is found on the surface of all B220+ B cells found in spleen. BAFF-R is expressed on activated/memory subsets of T-cells and is important for splenic B cell maturation and survival and is a major mediator of BAFF- dependent costimulatory responses in peripheral B and T cells. BAFF-R can cause a high level of autoimmune disease and a defect in BAFF-R will cause a decrease in generating mature B-Cells The ligand for the BAFF-R is BAFF also known as BLyS.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio7H22-E16 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio7H22-E16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ng LG, Ng CH, Woehl B, Sutherland AP, Huo J, Xu S, Mackay F, Lam KP. BAFF costimulation of Toll-like receptor-activated B-1 cells. Eur J Immunol. 2006 Jul;36(7):1837-46. (7H22-E16, FC Pubmed)

Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004 Jul 15;173(2):807-17.(7H22-E16, FC PubMed)

Thompson JS, Bixler SA, Qian F, Vora K, Scott ML, Cachero TG, Hession C, Schneider P, Sizing ID, Mullen C, Strauch K, Zafari M, Benjamin CD, Tschopp J, Browning JL, Ambrose C. BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF. Science. 2001 Sep 14;293(5537):2108-11

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Cat. 51-4301 Alexa Fluor® 647 Rat IgG1 Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.