Product Information

Contents: Alexa Fluor® 647 anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)
Catalog Number: 51-5898
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAR-1
Isotype: Armenian Hamster IgG

Staining of in vitro derived mast cells with Alexa Fluor® 647 Armenian Hamster IgG Iso Cntrl (cat. 51-4888) (open histogram) or Alexa Fluor® 647 anti-mouse FceRIa (MAR-1) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5898	FITC anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	518	FC
12-5898	PE anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	575	FC
51-5898	Alexa Fluor® 647 anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	633	668	FC
14-5898	Affinity Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC IH/F IP WB
16-5898	Functional Grade* Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC
13-5898	Biotin anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MAR-1 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MAR-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MAR-1 antibody has been pre-titrated and tested by flow cytometric of mouse mast cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K. Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18105-10. Epub 2005 Dec 5.(mar-1, FC,PubMed)

Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H. Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood. 2007 Aug 1;110(3):913-20. Epub 2007 Apr 4 (Mar-1, FC)

Yamaguchi M., K. Hirai, A. Komiya, M. Miyamasu, Y. Furumoto, R. Teshima, K. Ohta, Y. Morita, S. J. Galli, C. Ra, K. Yamamoto. (2001) Regulation of Mouse Mast Cell Surface Fc epsilon RI expression by dexamethasone. Int Immunol 13(7):843-51.

Related Products

Cat. 51-4888 Alexa Fluor® 647 Armenian Hamster IgG Isotype Control (clone eBio299Arm)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.