Product Information

Contents: Alexa Fluor® 647 anti-mouse Ly-49H (Ly49H)
Catalog Number: 51-5886
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3D10
Isotype: Mouse IgG1

Staining of C57Bl/6 splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) (left) or PE anti-mouse Ly-49A/D (cat. 12-5783) (right) and 0.5 μg of Alexa Fluor® 647 anti-mouse Ly-49H (3D10). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5886	Affinity Purified anti-mouse Ly-49H (Ly49H)	N/A	N/A	FA FC IP
13-5886	Biotin anti-mouse Ly-49H (Ly49H)	N/A	N/A	FC
11-5886	Fluorescein isothiocyanate (FITC) anti-mouse Ly-49H (Ly49H)	488	518	FC
51-5886	Alexa Fluor® 647 anti-mouse Ly-49H (Ly49H)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 3D10 recognizes Ly49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins. It has been shown that there is preferential staining of Ly49H with Ly49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 3D10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 3D10 antibody has been tested by flow cytometric analysis of C57BL/6 mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brown MG, Dokun AO, Heusel JW, Smith HR, Beckman DL, Blattenberger EA, Dubbelde CE, Stone LR, Scalzo AA, Yokoyama WM. Vital involvement of a natural killer cell activation receptor in resistance to viral infection. Science. 2001 May 4;292(5518):934-7. (3D10, FA PubMed)

Caraux A, Kim N, Bell SE, Zompi S, Ranson T, Lesjean-Pottier S, Garcia-Ojeda ME, Turner M, Colucci F. Phospholipase C-gamma2 is essential for NK cell cytotoxicity and innate immunity to malignant and virally infected cells. Blood. 2006 Feb 1;107(3):994-1002. Epub 2005 Oct 4. (3D10, FC PubMed)

Smith HR, Chuang HH, Wang LL, Salcedo M, Heusel JW, Yokoyama WM. Nonstochastic coexpression of activation receptors on murine natural killer cells. J Exp Med. 2000 Apr 17;191(8):1341-54. (3D10, FC, IP, FA, PubMed)

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Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.