mouse NKG2D (CD314)

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Contents: Alexa Fluor® 647 anti-mouse NKG2D / CD314 (blocking)
Catalog Number: 51-5880
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MI-6 (MI6)
Isotype: Rat IgG2a, λ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
51-5880-80	25 ug	$115
51-5880-82	100 ug	$225

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with PE anti-mouse pan-NK (DX5) (cat. 12-5971) and 0.5 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or 0.5 μg of Alexa Fluor® 647 anti-mouse NKG2D (MI-6) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-5880	Biotin anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
14-5880	Affinity Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
16-5880	Functional Grade* Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
51-5880	Alexa Fluor® 647 anti-mouse NKG2D / CD314 (blocking)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MI-6 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1α, -β, -γ, -δ, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1⁺ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MI-6 (MI6) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MI-6 (MI6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Jamieson AM, Diefenbach A, McMahon CW, Xiong N, Carlyle JR, Raulet DH. The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity. 2002 Jul;17(1):19-29. Erratum in: Immunity. 2004 Jun;20(6):799. (MI-6, FC, FA, PubMed)

Diefenbach A, Hsia J, Hsiung M, Raulet D. 2003. A novel ligand for the NKG2D receptor activates NK cells and macrophages and induces tumor immunity. Eur. J. Immunol. 33(2):381 - 391.

Lodoen M, Ogasawara K, Hamerman JA, Arase H, Houchins JP, Mocarski ES, Lanier LL. 2003. NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules. J Exp Med. 197(10):1245-53.

Cerwenka A, Baron JL, Lanier LL. 2001. Ectopic expression of retinoic acid early inducible-1 gene (RAE-1) permits natural killer cell-mediated rejection of a MHC class I-bearing tumor in vivo. Proc Natl Acad Sci U S A. 98(20):11521-6.

Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. 2000. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 12(6):721-7.

Related Products

Cat. 51-4321    Alexa Fluor® 647 Rat IgG2a Isotype Control
Cat. 12-5873    PE anti-mouse NKG2D / CD314 (blocking) (clone C7)
Cat. 13-5873    Biotin anti-mouse NKG2D / CD314 (blocking) (clone C7)
Cat. 14-5873    Affinity Purified anti-mouse NKG2D / CD314 (blocking) (clone C7)
Cat. 16-5873    Functional Grade Purified anti-mouse NKG2D / CD314 (blocking) (clone C7)
Cat. 12-5882    PE anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 13-5882    Biotin anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 14-5882    Affinity Purified anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 16-5882    Functional Grade Purified anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 17-5882    APC anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 25-5882    Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NKG2D / CD314 (blocking) (clone CX5)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.