Contents: Alexa FluorŽ 647 anti-mouse Vβ10 Vβa haplotype TCR
Catalog Number: 51-5807
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio8.48 (8.48)
Isotype: Mouse IgG1, κ
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Staining of SJL splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.5 μg of Alexa FluorŽ 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or 0.5 μg of Alexa FluorŽ 647 anti-mouse Vβ10 Vβa haplotype TCR (eBio8.48) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The eBio8.48 monoclonal antibody reacts with Vβ10 in mice carrying the Vβ
a haplotype of the TCR β chain. The majority of T cells express a TCR composed of a β chain and α chain, both of which are produced by a series of somatic DNA rearrangement events which join V, D and J segments, or in the case of TCR α, just V and J segments, to a TCR constant region. Murine TCR polymorphisms have been defined for Vβ as well as Vα genes based on RFLP analyses which has divided mouse strains into three Vβ haplotypes (Vβ
a, Vβ
b and Vβ
c), and at least four Vα haplotypes. While most mouse strains carry the Vβ
b haplotype, strains that are known to carry the Vβ
a haplotype include SWR, SJL, C57L, and C57BR mice.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio8.48 (8.48) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio8.48 (8.48) antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Smith LR, Plaza A, Singer PA, Theofilopoulos AN.
Coding sequence polymorphisms among V beta T cell receptor genes.
J Immunol. 1990 Apr 15;144(8):3234-7.
Behlke MA, Chou HS, Huppi K, Loh DY.
Murine T-cell receptor mutants with deletions of beta-chain variable region genes.
Proc Natl Acad Sci U S A. 1986 Feb;83(3):767-71.
Haqqi TM, Banerjee S, Anderson GD, David CS.
RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes.
J Exp Med. 1989 Jun 1;169(6):1903-9.
Related Products
Cat.
51-4714 Alexa FluorŽ 647 Mouse IgG1, K Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
