Contents: Alexa FluorŽ 647 anti-mouse GP49 receptor
Catalog Number: 51-5784
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: H1.1
Isotype: Armenian Hamster IgG
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Staining of BALB/c bone marrow cells with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 0.5 μg of Alexa FluorŽ 647 Armenian Hamster IgG Iso Cntrl (cat. 51-4888) (left) or 0.5 μg of Alexa FluorŽ 647 anti-mouse GP49 receptor (H1.1) (right). Total viable cells were used for analysis.
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Description
The monoclonal antibody H1.1 recognizes gp49, a receptor with structural similarities to the PIR (paired Ig-activating receptors) and ILT/KIR(killer inhibitory receptors) family of proteins. Two closely linked genes on mouse chromosome 10 encode the related proteins gp49A and gp49B. These two receptors are almost 90% identical in their extracellular regions but differ in their cytoplasmic domains; gp49B contains an ITAM domain while gp49A does not and is extremely short.
Expression of gp49 was originally identified on mast and LAK (IL-2 activated NK) cells. Expression on LAK cells has been shown to be predominantly gp49B with only a small amount of gp49A. Additionally gp49 is found on myeloid cells. Detailed function is not known but gp49B can inhibit cellular activation. The monoclonal antibody H1.1 recognizes both gp49A and gp49B.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This H1.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This H1.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Gu X, Laouar A, Wan J, Daheshia M, Lieberman J, Yokoyama WM, Katz HR, Manjunath N.The gp49B1 inhibitory receptor regulates the IFN-gamma responses of T cells and NK cells. J Immunol. 2003 Apr 15;170(8):4095-101.(H1.1, FC,
PubMed)
Wang LL, Chu DT, Dokun AO, Yokoyama WM. Inducible expression of the gp49B inhibitory receptor on NK cells. J Immunol. 2000 May 15;164(10):5215-20.(H1.1, FC, FA
PubMed)
Related Products
Cat.
51-4888 Alexa FluorŽ 647 Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart