Product Information

Contents: Alexa Fluor® 647 Mouse Ly49D (Ly49D)
Catalog Number: 51-5782
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio4E5 (4E5)
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-5782	Alexa Fluor® 647 anti-mouse Ly49D (Ly49D)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio4E5 reacts with Ly49D, an activating member of the Ly49 family of NK cell receptors. The receptors form homodimers which bind MHC Class I alloantigens. The activating Ly49 receptors (Ly49D and Ly49H) do not contain any immune receptor tyrosine-based inhibitory motif (ITAM) in their cytoplasmic domains unlike inhibitory Ly49 family members. Alternatively, Ly49 molecules have been shown to associate with a 12-kDa homodimeric protein, DAP12, that contains an immunoreceptor tyrosine-based activation motif critical for positive signaling by these receptors. Ly49D can mobilize intracellular 2+Ca and mediate reverse antibody-dependent cellular cytotoxicity in the presence of specific antibody. Expression of family members is independent therefore several Ly49 proteins can be expressed on the same cells. Expression of Ly49D is strain dependent: on subsets of natural killer (NK) cells in C57BL/6, CB6F-1 and SJL, while BALB/c, DBA/2, AKR, and CBA/J are negative. In 129/J mice, eBio4E5 also recognizes Ly49O and Ly49V and LY49R.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This eBio4E5 (4E5) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Makrigiannis AP, Pau AT, Saleh A, Winkler-Pickett R, Ortaldo JR, Anderson SK. Class I MHC-binding characteristics of the 129/J Ly49 repertoire. J Immunol. 2001 Apr 15;166(8):5034-43. (4E5, FC and IP, PubMed)

Ortaldo, J.R., et al. 1998. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J. Immunol. 160: 1158 - 1165.
Yokoyama, W.M., and W.E. Seaman. 1993. The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: The NK gene complex. Annu. Rev. Immunol. 11: 613 - 635.
Raulet, D.H., W. Held, I. Correa, J.R. Dorfman, M.-F. Wu, and L. Corral. 1997. Specificity, tolerance and developmental regulation of natural killer cells defined by expression of class I-specific Ly49 receptors. Immunol. Rev. 155: 41 – 52.

Related Products

Cat. 51-4321    Alexa Fluor® 647 Rat IgG2a Isotype Control
Cat. 11-5781    Fluorescein isothiocyanate (FITC) anti-mouse Ly-49G2 (Ly49G2, LGL-1) (clone eBio4D11 (4D11))
Cat. 12-5783    Phycoerythrin (PE) anti-mouse Ly-49A/D (Ly49A/D) (clone eBio12A8 (12A8))

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.