Product Information

Contents: Alexa Fluor® 647 Mouse JAML (Junctional Adhesion Molecule-Like protein)
Catalog Number: 51-5666
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio4E10 (4E10)
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 lysed whole blood with FITC anti-mouse Gr-1 (RB6-8C5) (cat. 11-5931) and 0.25 μg of Alexa Fluor® 647 Armenian Hamster IgG Iso Cntrl (cat. 51-4888) (left) or 0.25 μg of Alexa Fluor® 647 Mouse JAML (Junctional Adhesion Molecule-Like) (right). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-5666	Alexa Fluor® 647 anti-mouse JAML (Junctional Adhesion Molecule-Like)	633	668	FC
14-5666	Affinity Purified anti-mouse JAML (Junctional Adhesion Molecule-Like)	N/A	N/A	FC IP

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as γδ T cell lines, and can provide co-stimulatory signals for cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4E10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zen K, Liu Y, McCall IC, Wu T, Lee W, Babbin BA, Nusrat A, Parkos CA. Neutrophil migration across tight junctions is mediated by adhesive interactions between epithelial coxsackie and adenovirus receptor and a junctional adhesion molecule-like protein on neutrophils. Mol Biol Cell. 2005 Jun;16(6):2694-703. (PubMed)

Moog-Lutz C, Cave-Riant F, Guibal FC, Breau MA, Di Gioia Y, Couraud PO, Cayre YE, Bourdoulous S, Lutz PG. JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells. Blood. 2003 Nov 1;102(9):3371-8. (PubMed)

Related Products

Cat. 51-4888 Alexa Fluor® 647 Armenian Hamster IgG Isotype Control (clone eBio299Arm)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.