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Anti-Mouse JAML Alexa Fluor® 647
Also known as: Junctional Adhesion Molecule-Like, AMICA
RUO: For Research Use Only
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 |  | Staining of C57BL/6 lysed whole blood cells with Anti-Mouse Ly-6G (Gr-1) FITC (cat. 11-5931) and 0.25 µg of Armenian Hamster IgG Isotype Control Alexa Fluor® 647 (left) or 0.25 µg of Mouse JAML Alexa Fluor® 647 (right). Cells in the granulocyte gate were used for analysis. |
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Contents: Anti-Mouse JAML Alexa Fluor® 647
Catalog Number: 51-5666
Concentration: 0.2 mg/ml
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: eBio4E10 (4E10)
Host/Isotype: Armenian Hamster IgG
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| Product Options |
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| Cat. No. |
Size |
Price |
Add Qty to Cart |
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
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| Available Formats |
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| Cat. No. | Format | Excite
(nm) | Emit
(nm) | Reported Applications | Regulatory Status |
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| 14-5666 |
Anti-Mouse JAML Purified |
N/A |
N/A |
FC IP |
RUO |
| 51-5666 |
DISCONTINUED - Anti-Mouse JAML Alexa Fluor® 647 |
633 |
668 |
FC |
RUO |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products. |
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| Description |
| The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as γδ T cell lines, and can provide co-stimulatory signals for cell activation. |
| Applications Reported |
| For research use only, not for diagnostic or therapeutic use. This eBio4E10 antibody has been reported for use in flow cytometric analysis. |
| Applications Tested |
| This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| References |
Zen K, Liu Y, McCall IC, Wu T, Lee W, Babbin BA, Nusrat A, Parkos CA. Neutrophil migration across tight junctions is mediated by adhesive interactions between epithelial coxsackie and adenovirus receptor and a junctional adhesion molecule-like protein on neutrophils. Mol Biol Cell. 2005 Jun;16(6):2694-703. (PubMed)
Moog-Lutz C, Cave-Riant F, Guibal FC, Breau MA, Di Gioia Y, Couraud PO, Cayre YE, Bourdoulous S, Lutz PG. JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells. Blood. 2003 Nov 1;102(9):3371-8. (PubMed) |
| Legal |
| Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc. |
