Product Information

Contents: Alexa Fluor® 647 anti-mouse FR4 (Folate receptor 4, FBP, FRδ)
Catalog Number: 51-5445
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio12A5
Isotype: Rat IgG2b, κ

Left: Staining of mouse splenocytes with PE-Cy7 anti-mouse CD25 (PC61.5) (cat. 25-0251) and 0.25 µg of AlexaFluor647 anti-mouse FR4. Right: analysis of gated CD4+CD25+ cells stained with AlexaFluor 647 isotype control (cat. 51-4031)(open) or AlexaFluor647 anti-mouse FR4 (colored).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5445	Affinity Purified anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	N/A	N/A	FC IP
13-5445	Biotin anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	N/A	N/A	FC
11-5445	Fluorescein isothiocyanate (FITC) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	518	FC
12-5445	Phycoerythrin (PE) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	575	FC
25-5445	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	760	FC
51-5445	Alexa Fluor® 647 anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR δ and FBP3 (folate binding protein3). FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naïve T cells. FR4^hi CD25^hi cells are natural Tregs with high levels of Foxp3. FR4^hi CD25^lo cells are identified as central memory T cells which upon stimulation can proliferate and produce large amounts of IL-2. In contrast FR4^lo CD25^hi secrete proinflammatory cytokines such as IFNγ and IL-17 and have been characterized as effector memory T cells.

Based on co-staining studies, the epitopes recognized by eBioTH6 (cat 51-5446) and eBio12A5 are different thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio12A5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio12A5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Elnakat H, Ratnam M. Distribution, functionality and gene regulation of folate receptor isoforms: implications in targeted therapy. Adv Drug Deliv Rev. 2004 Apr 29;56(8):1067-84.

Spiegelstein O, Eudy JD, Finnell RH. Identification of two putative novel folate receptor genes in humans and mouse. Gene. 2000 Nov 27;258(1-2):117-25.

Sugimoto N, Oida T, Hirota K, Nakamura K, Nomura T, Uchiyama T, Sakaguchi S. Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis. Int Immunol. 2006 Aug;18(8):1197-209.

Yamaguchi T, Hirota K, Nagahama K, Ohkawa K, Takahashi T, Nomura T, Sakaguchi S. Control of immune response by antigen-specific regulatory T cells expressing the folate receptor. Immunity July 2007 ePub (eBio12A5 FC, IP)

Related Products

Cat. 11-0041    FITC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-0251    Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (clone PC61.5)
Cat. 51-4031    Alexa Fluor® 647 Rat IgG2b Isotype Control
Cat. 16-5446    Functional Grade Purified anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd) (clone eBioTH6)
Cat. 00-5523    eBioscience Foxp3 Staining Buffer Set

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.