Contents: Alexa FluorŽ 647 anti-mouse 3G11 sialoganglioside antigen
Catalog Number: 51-5443
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio3G11 (SM3G11, 3G11)
Isotype: Mouse IgM, κ
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Staining of C57Bl/6 splenocytes with FITC anti-mouse CD4 (GK1.5) (cat. 11-0041) and staining buffer (autofluorescence) (left) or 0.25 μg of Alexa FluorŽ 647 anti-mouse 3G11 (SM3G11) (right). Total viable cells were used for analysis.
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Description
The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naīve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3ε, suggesting close proximity of these two proteins.
Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio3G11 (SM3G11, 3G11) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio3G11 (SM3G11, 3G11) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hayakawa K, Hardy RR.
Murine CD4+ T cell subsets defined.
J Exp Med. 1988 Nov 1;168(5):1825-38. (
3G11, FC,
PubMed)
Zhang GX, Yu S, Calida D, Zhao Z, Gran B, Kamoun M, Rostami A.
Loss of the surface antigen 3G11 characterizes a distinct population of anergic/regulatory T cells in experimental autoimmune encephalomyelitis.
J Immunol. 2006 Mar 15;176(6):3366-73. (
3G11, FC,
PubMed)
Ernst DN, Hobbs MV, Torbett BE, Glasebrook AL, Rehse MA, Bottomly K, Hayakawa K, Hardy RR, Weigle WO.
Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice.
J Immunol. 1990 Sep 1;145(5):1295-302. (
3G11, FC,
PubMed)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart