Staining of mouse PECs with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 0.5 μg of Alexa FluorŽ 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or Alexa FluorŽ 647 anti-mouse Galectin-3 (M3/38) (right). Total viable cells were used for analysis.
Contents: Alexa FluorŽ 647 anti-mouse Galectin-3 / Mac-2 Catalog Number: 51-5301 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Clone: eBioM3/38 (M3/38) Host/Isotype: Rat IgG2a, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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51-5301-80
25 ug
51-5301-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.
This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioM3/38 (M3/38) antibody has been tested by intracellular staining of thyoglycolated elicited peritoneal cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ho MK, Springer TA. 1982. MAC-2, A Novel 32000 Mr Mouse Macrophage Subpopulation-Specific Antigen Defined by Monoclonal Antibodies. J. Immunol. 128(3):1221-1228.
Joo G-H, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a b-galactoside-binding protein in activated T lymphocytes J. Leukoc. Biol. 69: 555564; 2001.
Oliveira FL, Fraza P, Chammas R, Hsu DK, Liu FT, Borojevic R, Takiya CM,
El-Cheikh MC, Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice J. Leukoc. Biol. 82: ePub; 2007
Springer TA, 1981. Monoclonal Antibody Analysis of Complex Biological Systems. J. Biol Chem. 256(8):3833-3839.
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
