Contents: Alexa Fluor® 647 anti-mouse PDCA-1 (BST2, CD317)
Catalog Number: 51-3171
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio129c (129c)
Isotype: Rat IgG2b
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Staining of SJL splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and Alexa Fluor® 647 anti-mouse PDCA-1 (BST2, CD317) (left) or PE anti-mouse CD11c (cat. 12-0114) and Alexa Fluor® 647 anti-mouse PDCA-1 (BST2, CD317) (right). Cells in the large forward scatter population were used for analysis.
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Description
The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represent sa very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8.
The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio129c (129c) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio129c (129c) antibody has been tested by flow cytometric analysis of SJL splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Blasius A.L., E. Giurisat, M. Cella, R.D. Schreiber, A.S. Shaw, M. Colonna. 2006. Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naïve Mouse, but a Promiscuous Cell Surface Antigen Following IFN Stimulation. J Immunol. 177:3260-3265
Related Products
Cat.
51-3172 Alexa Fluor® 647 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) (clone eBio927)
Cat.
51-4031 Alexa Fluor® 647 Rat IgG2b Isotype Control
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart