Product Information

Contents: Alexa Fluor® 647 anti-mouse MIG
Catalog Number: 51-3009
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MIG-2F5.5
Isotype: Armenian Hamster IgG

Staining of LPS-stimulated (left) and IFN-g-stimulated (right) BALB/c thioglycolate PEC with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 0.125 μg of Alexa Fluor® 647 anti-mouse MIG (MIG-2F5.5). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-3009	Phycoerythrin (PE) anti-mouse MIG (CXCL9, monokine induced by IFN-g)	488	575	IC Flow
51-3009	Alexa Fluor® 647 anti-mouse MIG (CXCL9, monokine induced by IFN-g)	633	668	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN-g (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN-g, but not other activators such as LPS or IFN-a. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MIG-2F5.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This MIG-2F5.5 antibody has been tested by intracellular staining followed by flow cytometric analysis of IFN-g stimulated thioglycolate PEC. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lu H, et al. 2007. Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli. Protein Expr Purif. 2007 Sep;55(1):132-8. Epub 2007 Apr 14.

Wong P, et al. 1994. A unique palindromic element mediates gamma interferon induction of mig gene expression. Mol. Cell. Biol. 14: 914-922

F Liao, et al. 1995. Human Mig chemokine: biochemical and functional characterization. J. Exp. Med. 182: 1301-1314.

Klunker S, et al. 2003. A Second Step of Chemotaxis After Transendothelial Migration: Keratinocytes Undergoing Apoptosis Release IFN-{gamma}-Inducible Protein 10, Monokine Induced by IFN-{gamma}, and IFN-{gamma}-Inducible {alpha}-Chemoattractant for T Cell Chemotaxis Toward Epidermis in Atopic Dermatitis. J Immunol 171: 1078-1084

Related Products

Cat. 51-4888 Alexa Fluor® 647 Armenian Hamster IgG Isotype Control (clone eBio299Arm)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.