Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
Description
The 15B8 monoclonal antibody reacts with human and mouse ß-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, β-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, β-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, β-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). β-catenin is expressed in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt-β-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This 15B8 antibody has been pre-titrated and tested by intracellular flow cytometric analysis of the Jurkat cell line. This can be used at 5 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 ĩL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Xu M, Sharma A, Hossain MZ, Wiest DL, Sen JM. Sustained expression of pre-TCR induced beta-catenin in post-beta-selection thymocytes blocks T cell development. J Immunol. 2009 Jan 15;182(2):759-65.
Staal FJ, Sen JM. The canonical Wnt signaling pathway plays an important role in lymphopoiesis and hematopoiesis. Eur J Immunol. 2008 Jul;38(7):1788-94. Review.
Xu, Y, Banerjee D, Huelsken J, Birchmeier W, and Sen JM. Deletion of beta-catenin impairs T cell development. Nat. Immunol. 2003 4:1177-1182.
Hoschuetzky H, Aberle H, Kemler R. Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor.
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
