Product Information

Contents: Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Alloantigen)
Catalog Number: 51-2441
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio244F4
Isotype: Rat IgG2a

Left: C57BL/6 splenocytes were cultured in IL-2 for 5 days and stained with APC DX5 (CD49b) and 0.06ug AlexaFluor 647 eBio244F4 (4F4).
Right: C57BL/6 splenocytes were stained with FITC PK136 (NK1.1) and 0.06ug AlexaFluor 647 eBio244F4.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-2441	Fluorescein isothiocyanate (FITC) anti-mouse CD244.2 (2B4 B6 Alloantigen)	488	518	FC
12-2441	Phycoerythrin (PE) anti-mouse CD244.2 (2B4 B6 Alloantigen)	488	575	FC
51-2441	Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Alloantigen)	633	668	FC
14-2441	Affinity Purified anti-mouse CD244.2 (2B4 B6 Alloantigen)	N/A	N/A	FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2–driven expansion and activation of murine NK cells.

Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.

eBio244F4 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. The epitope recognized by eBio244F4 is different from that of 2B4; therefore these antibodies do not cross-block the binding to the respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio244F4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio244F4 antibody has been tested by flow cytometric analysis of IL-2-stimulated mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Garni-Wagner BA, Purohit A, Mathew PA, Bennett M, Kumar V. A novel function-associated molecule related to non-MHC-restricted cytotoxicity mediated by activated natural killer cells and T cells. J Immunol. 1993 Jul 1;151(1):60-70.

Kumaresan PR, Huynh VT, Mathew PA. Polymorphism in the 2B4 gene of inbred mouse strains. Immunogenetics. 2000 Jul;51(8-9):758-61

Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wulfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V. Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15;107(8):3181-8.

Mathew PA, Garni-Wagner BA, Land K, Takashima A, Stoneman E, Bennett M, Kumar V. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993 Nov 15;151(10):5328-37.

Related Products

Cat. 12-0481    Phycoerythrin (PE) anti-mouse CD48 (BCM1) (clone HM48-1)
Cat. 51-4321    Alexa Fluor® 647 Rat IgG2a Isotype Control
Cat. 11-5941    FITC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) (clone PK136)
Cat. 12-5971    PE anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2) (clone DX5)

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