Product Information

Contents: Alexa Fluor® 647 anti-mouse CD207 (Langerin)
Catalog Number: 51-2073
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioRMUL.2
Isotype: Rat IgG2a, κ

Intracellular staining of 293T cells transiently transfected with mouse CD207 with staining buffer (autofluorescence) (left) or 0.5 μg of Alexa Fluor® 647 anti-mouse CD207 (eBioRMUL.2) (right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-2073	Alexa Fluor® 647 anti-mouse CD207 (Langerin)	633	668	IC Flow
53-2073	Alexa Fluor® 488 anti-mouse CD207 (Langerin)	488	519	IC Flow
14-2073	Affinity Purified anti-mouse CD207 (Langerin)	N/A	N/A	IC Flow IHC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

RMUL.2 is a monoclonal antibody that reacts with the predicted extracellular domain of mouse Langerin (CD207). Mouse Langerin(CD207) is expressed in a distinct subset of Dendritic cells called Langerhans cells (LC). Mouse Langerin is a 48kDa C-type lectin putative transmembrane protein that likely plays a role in antigen recognition and uptake. Langerhans cells are located in the epidermis and upon activation, reduce Langerin expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse Langerin in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of Langerin cDNA into fibroblast cell lines.

Western Blotting with RMUL.2 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse Langerin cDNA. Cross reactivity of this antibody with other proteins has not been determined. Preliminary data from mouse Langerin cDNA transfected cells suggests that RMUL.2 can be used for intracellular flow cytometric analysis. We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface. In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining when compared to a polyclonal antibody obtained from a different supplier.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioRMUL.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioRMUL.2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse CD207-transfected 293T cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Stoitzner P, Holzman , McLellan AD, Ivarsson L, Stossel H, Kapp M, Kammerer U, Douillard P, Kampgen E, Koch F, Saeland S, Romani N. Visualization and characterization of migratory Langerhans cells in murine skin and lymph nodes by antibodies against Langerin/CD207. J Invest Dermatol. 2003 Feb;120(2):266-74. .

Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol. 2002 Jan 15;168(2):782-92.

Molinero LL, Zhou P, et al. 2008. Epidermal Langerhans cells promote skin allograft rejection in mice with NF-kappa B-impaired T cells. Am J Transplant. 8(1):21-31. (eBioRMUL.2, IC flow, PubMed)

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Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.