Product Information

Contents: Alexa Fluor® 647 anti-mouse CD201 (EPCR, endothelial protein C receptor)
Catalog Number: 51-2011
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBiomRCR-16 (mRCR-16)
Isotype: Rat IgG2a, κ

Staining of bEnd.3 cells with 0.125 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (open histogram) or 0.125 μg of Alexa Fluor® 647anti-mouse CD201 (eBiomRCR-16) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-2011	Alexa Fluor® 647 anti-mouse CD201 (EPCR, endothelial protein C receptor)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioRCR-16 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.

Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBiomRCR-16 (mRCR-16) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBiomRCR-16 (mRCR-16) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Balazs AB, Fabian AJ, Esmon CT, Mulligan RC. Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow. Blood. 2006 Mar 15;107(6):2317-21. Epub 2005 Nov 22.

Fukudome K, Esmon CT. Molecular cloning and expression of murine and bovine endothelial cell protein C/activated protein C receptor (EPCR). The structural and functional conservation in human, bovine, and murine EPCR. J Biol Chem. 1995 Mar 10;270(10):5571-7.

Li W, Zheng X, Gu JM, Ferrell GL, Brady M, Esmon NL, Esmon CT. Extraembryonic expression of EPCR is essential for embryonic viability. Blood. 2005 Oct 15;106(8):2716-22.

Related Products

Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.