Product Information

Contents: Alexa Fluor® 647 anti-mouse gp130 (CD130)
Catalog Number: 51-1302
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: KGP130
Isotype: Rat IgG2a, k

Staining of BALB/c splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.25 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or 0.25 μg of Alexa Fluor® 647 anti-mouse gp130 (KGP130) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-1302	Alexa Fluor® 647 anti-mouse gp130 (CD130)	633	668	FC
14-1302	Affinity Purified anti-mouse gp130 (CD130)	N/A	N/A	FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The KGP130 monoclonal antibody reacts with mouse gp130, also known as CD130. gp130 is a 130 kD Type-I transmembrane glycoprotein containing a 597 amino acid extracellular domain, a single transmembrane domain, and a 277 amino acid cytoplasmic domain. gp130 is a subunit of several heterodimeric cell-surface receptors, including receptors for IL-6, IL-11, IL-27, Oncostatin M, and Leukemia Inhibitory Factor (LIF). The gp130 protein has also been found to exist in a soluble form, which is capable of inhibiting IL-6 signaling. gp130 is expressed mainly on T cells, monocytes, endothelial cells, activated B cells, and plasma cells, and is expressed at lower levels on most leukocytes and epithelial cells. In response to ligand binding, gp130 becomes tyrosine phosphorylated, leading to activation of several signaling pathways including the PI3 kinase, Ras-MAPk and Stat pathways.

Preincubation of mouse splenic T cells with IL-6 is capable of blocking the interaction of KGP130 with gp130, suggesting that the KGP130 binding site lies near the IL-6 interaction domain. Reactivity of KGP130 towards human gp130 has not been observed.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This KGP130 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This KGP130 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Arzt E. gp130 cytokine signaling in the pituitary gland: a paradigm for cytokine-neuro-endocrine pathways. J Clin Invest. 2001 Dec;108(12):1729-33. (PubMed)

Bravo J, Heath JK. Receptor recognition by gp130 cytokines. EMBO J. 2000 Jun 1;19(11):2399-411. (PubMed)

Wang XJ, Taga T, Yoshida K, Saito M, Kishimoto T, Kikutani H. gp130, the cytokine common signal-transducer of interleukin-6 cytokine family, is downregulated in T cells in vivo by interleukin-6. Blood. 1998 May 1;91(9):3308-14. (PubMed)

Related Products

Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.