Product Information

Contents: Alexa Fluor® 647 anti-human CD107a (LAMP-1)
Catalog Number: 51-1079
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioH4A3
Isotype: Mouse IgG1, κ

Intracellular staining of Jurkat cells with Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (open histogram) or Alexa Fluor® 647 anti-human CD107a (H4A3) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1079	Fluorescein isothiocyanate (FITC) anti-human CD107a (LAMP-1)	488	518	IC Flow
12-1079	Phycoerythrin (PE) anti-human CD107a (LAMP-1)	488	575	IC Flow
15-1079	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD107a (LAMP-1)	488	670	IC Flow
51-1079	Alexa Fluor® 647 anti-human CD107a (LAMP-1)	633	668	IC Flow
53-1079	Alexa Fluor® 488 anti-human CD107a (LAMP-1)	488	519	IC Flow
14-1079	Affinity Purified anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow IHC WB
13-1079	Biotin anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as LAMP-1 (lysosomal-associated membrane protein 1). CD107a, an approximately 110kDa protein, is highly glycosylated, and along with LAMP-2 (CD107b) is estimated to contribute to approximately 50% of all proteins of the lysosomal membrane. CD107a is expressed intracellularly on many cell types, and on the surface of activated platelets and T cells, as well as some cancer cells. It is reported that surface CD107a binds to CD62E (E-Selectin).

It is recommended to use the brightest fluorochromes (cat. 12-1079) for staining for rather than purified and biotinylated formats. Please see other formats for data comparison.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Sarafian V, Jans R, Poumay Y. Expression of lysosome-associated membrane protein 1 (LAMP-1) and galectins in human keratinocytes is regulated by differentiation. Arch. Dermatol. Res. 2006 Jul; 298(2):73-81. (H4A3, IHC, WB, PubMed)

Huynh C, Andrews NW. The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis. EMBO Rep. 2005 Sep;6(9):843-7. (H4A3, FC, IHC, PubMed)

Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brule F, Castronovo V, Wattiaux R, Coninck SW. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells. Int. J. Cancer. 1998 Jan; 75(1):105-111. (H4A3, FC, IHC, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

Related Products

Cat. 51-1071 Alexa Fluor® 647 anti-mouse CD107a (LAMP-1) (clone eBio1D4B (1D4B))
Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.