Product Information

Contents: Alexa Fluor® 647 anti-human CD107b (LAMP-2)
Catalog Number: 51-1078
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioH4B4 (H4B4)
Isotype: Mouse IgG1, κ

Intracellular staining of Jurkat cells with Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (open histogram) or Alexa Fluor® 647 anti-human CD107b (H4B4) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1078	Fluorescein isothiocyanate (FITC) anti-human CD107b (LAMP-2)	488	518	IC Flow
51-1078	Alexa Fluor® 647 anti-human CD107b (LAMP-2)	633	668	IC Flow
53-1078	Alexa Fluor® 488 anti-human CD107b (LAMP-2)	488	519	IC Flow
14-1078	Affinity Purified anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow
13-1078	Biotin anti-human CD107b (LAMP-2)	N/A	N/A	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as LAMP-2 (lysosomal-associated membrane protein 2). CD107b, an approximately 105 kDa type I transmembrane protein, is highly glycosylated, and along with LAMP-1 (CD107a) is estimated to contribute to approximately 50% of all proteins of the lysosomal membrane. CD107b is expressed intracellularly on many cell types, and on the surface of activated platelets and T cells, as well as some cancer cells. Genetic deficiency of both CD107a and CD107b results in embryonic lethality. Loss of either CD107a or CD107b alone is not embryonic lethal, however the loss of CD107b causes a more severe phenotype. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioH4B4 (H4B4) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioH4B4 (H4B4) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-910. (H4B4, FC, IHC, PubMed)

Kannan K, Stewart RM, Bounds W, Carlsson SR, Fukuda M, Betzing KW, Holcombe RF. Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium. Cell Immunol. 1996;171(1):10-19. (H4B4, FC, IHC, PubMed)

Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85-95.

Carlsson SR, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988 Dec 15;263(35):18911-9.

Grutzkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8.

Eskelinen EL, Illert AL, Tanaka Y, Schwarzmann G, Blanz J, Von Figura K, Saftig P. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002 Sep;13(9):3355-68.

Related Products

Cat. 51-1072 Alexa Fluor® 647 anti-mouse CD107b (LAMP-2) (clone eBioABL-93 (ABL-93))
Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.