Product Information

Contents: Alexa Fluor® 647 anti-mouse CD106 (VCAM-1)
Catalog Number: 51-1061
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 429
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with 0.25 μg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or 0.25 μg of Alexa Fluor® 647 anti-mouse CD106 (429) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1061	FITC anti-mouse CD106 (VCAM-1)	488	518	FC
51-1061	Alexa Fluor® 647 anti-mouse CD106 (VCAM-1)	633	668	FC
14-1061	Affinity Purified anti-mouse CD106 (VCAM-1)	N/A	N/A	FA FC IHC IP
16-1061	Functional Grade* Purified anti-mouse CD106 (VCAM-1)	N/A	N/A	FA FC
13-1061	Biotin anti-mouse CD106 (VCAM-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin α₄β₁ (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 429 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kinashi, T., Y. St. Pierre, et al. (1995).Expression of glycophosphatidylinositol-anchored and -non-anchored isoforms of vascular cell adhesion molecule 1 in murine stromal and endothelial cells. J Leukoc Biol 57(1): 168-73.

Related Products

Cat. 13-1060    Biotin anti-rat CD106 (VCAM-1) (clone eBioMR106 (MR106))
Cat. 14-1060    Affinity Purified anti-rat CD106 (VCAM-1) (clone eBioMR106 (MR106))
Cat. 16-1060    Functional Grade Purified anti-rat CD106 (VCAM-1) (clone eBioMR106 (MR106))
Cat. 51-4321    Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.