Product Information

Contents: Alexa Fluor® 647 anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)
Catalog Number: 51-0349
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4H11
Isotype: Mouse IgG1

Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or Alexa Fluor® 647 anti-human CD34 (4H11) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0349	Phycoerythrin (PE) anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	488	575	FC
25-0349	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	488	760	FC
51-0349	Alexa Fluor® 647 anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	633	668	FC
14-0349	Affinity Purified anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	N/A	N/A	FA FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated, extracellular, and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines and also by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage with neuraminidase, chymopapain and O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4H11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P. Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20. (4H11, FA, FC, PubMed)

Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE. Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34. Leukemia. 1988 Dec;2(12):793-803.

Baumheter S, Singer MS, Henzel W, Hemmerich S, Renz M, Rosen SD, Lasky LA. Binding of L-selectin to the vascular sialomucin CD34. Science. 1993 Oct 15;262(5132):436-8.

Related Products

Cat. 51-0341 Alexa Fluor® 647 anti-mouse CD34 (clone RAM34)
Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.