Product Information

Contents: Alexa Fluor® 647 anti-human mature macrophage marker (late-stage)
Catalog Number: 51-0115
Sizes: 25 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio25F9 (25F9)
Isotype: Mouse IgG1

Staining of monocytes from freshly isolated human peripheral blood cells (left) or cultured peripheral blood cells for 7 days with GM-CSF (right) with 0.25 μg of Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (open histogram) or Alexa Fluor® 647 anti-human mature macrophage marker (25F9) (colored histogram).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-0115	Alexa Fluor® 647 anti-human mature macrophage marker (late-stage)	633	668	FC
14-0115	Affinity Purified anti-human mature macrophage marker (late-stage)	N/A	N/A	FC IH/F IHC(Paraffin) IP WB
13-0115	Biotin anti-human mature macrophage marker (late-stage)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio25F9 (25F9) antibody has been pre-titrated and tested by flow cytometric analysis of cultured human macrophages. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

Related Products

Cat. 51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.