Contents: Alexa FluorŽ 647 anti-human mature macrophage marker (late-stage)
Catalog Number: 51-0115
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio25F9 (25F9)
Isotype: Mouse IgG1
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Staining of monocytes from freshly isolated human peripheral blood cells (left) or cultured peripheral blood cells for 7 days with GM-CSF (right) with 0.25 μg of Alexa FluorŽ 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (open histogram) or Alexa FluorŽ 647 anti-human mature macrophage marker (25F9) (colored histogram).
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Description
The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio25F9 (25F9) antibody has been pre-titrated and tested by flow cytometric analysis of cultured human macrophages. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
Related Products
Cat.
51-4714 Alexa FluorŽ 647 Mouse IgG1, K Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart