Contents: Alexa FluorŽ 647 anti-mouse CD9
Catalog Number: 51-0091
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioKMC8 (KMC8)
Isotype: Rat IgG2a, κ
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Staining of C57Bl/6 splenocytes with FITC anti-mouse/human CD11b (M1/70) (cat. 11-0112) and 0.5 μg of Alexa FluorŽ 647 Rat IgG2a Iso Cntrl (cat. 51-4321) (left) or 0.5 μg of Alexa FluorŽ 647 anti-mouse CD9 (KMC8) (right). Cells in the lymphocyte and monocyte gates were used for analysis.
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Description
The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naīve T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcγRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW. Related Articles, Links
Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.
J Exp Med. 1990 Feb 1;171(2):477-88. (
KMC8, mAb development,
PubMed)
Oritani K, Wu X, Medina K, Hudson J, Miyake K, Gimble JM, Burstein SA, Kincade PW.
Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures.
Blood. 1996 Mar 15;87(6):2252-61.
Tachibana I, Hemler ME.
Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance.
J Cell Biol. 1999 Aug 23;146(4):893-904. (
KMC8, WB, IP, FA,
PubMed)
Related Products
Cat.
51-4321 Alexa FluorŽ 647 Rat IgG2a Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart