Product Information

Contents: Alexa Fluor® 647 anti-mouse CD8a (Ly-2)
Catalog Number: 51-0081
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53-6.7
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 μg of Alexa Fluor® 647 anti-mouse CD8a (53-6.7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0081	Affinity Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC IH/F IP
16-0081	Functional Grade* Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FA FC
13-0081	Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC
11-0081	FITC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	518	FC
12-0081	PE anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	575	FC
15-0081	PE-Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	670	FC
17-0081	APC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	660	FC
19-0081	Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	670	FC
25-0081	PE-Cy7 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	760	FC
36-0081	Functional Grade* Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC IH/F
51-0081	Alexa Fluor® 647 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	668	FC
53-0081	Alexa Fluor® 488 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	519	FC
56-0081	Alexa Fluor® 700 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Cy5.5 Equivalent)	633	723	FC
45-0081	PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (PE-Cy5.5 replacement)	488	690	FC
93-0081	eFluor^TM 605^NC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	350-500	605	FC
95-0081	eFluor^TM 650^NC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	350-500	650	FC
47-0081	Allophycocyanin-eFluor^TM 780 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0081	eFluor^TM 450 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature αβ TCR T cells express CD8 αβ while γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53-6.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (53-6.7, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (53-6.7, in vivo depletion, PubMed)

Taylor JL, Ordway DJ, Troudt J, Gonzalez-Juarrero M, Basaraba RJ, Orme IM. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun. 2005 Aug;73(8):5189-93. (53-6.7, IHC frozen)

Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K. Beta2 integrins are required for skin homing of primed T cells but not for priming naïve T cells. J Clin Invest. 2002 Jan;109(2):183-92. (53-6.7, IHC frozen)

Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95.

Ledbetter, J. A. and L. A. Herzenberg. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90.

Related Products

Cat. 51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.