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Anti-Human CD4 Alexa Fluor® 647

Also known as: Leu-3, T4

RUO: For Research Use Only
human CD4 antibody, Alexa Fluor® 647 conjugateStaining of normal human peripheral blood cells with Anti-Human CD3 FITC (cat. 11-0037) and staining buffer (autofluorescence) (left) or Anti-Human CD4 Alexa Fluor® 647 (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD4 Alexa Fluor® 647
Catalog Number: 51-0048
Concentration: 5 μl (0.06 μg)/test
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: OKT4 (OKT-4)
Host/Isotype: Mouse IgG2b
 
Product Options
Cat. No. Size Price Add Qty to Cart
51-0048-41 25 tests
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51-0048-42 100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-0049 Anti-Human CD4 FITC (RPA-T4)
12-0049 Anti-Human CD4 PE (RPA-T4)
13-0049 Anti-Human CD4 Biotin (RPA-T4)
14-0049 Anti-Human CD4 Purified (RPA-T4)
15-0049 Anti-Human CD4 PE-Cy5 (RPA-T4)
16-0049 Anti-Human CD4 Functional Grade Purified (RPA-T4)
17-0049 Anti-Human CD4 APC (RPA-T4)
25-0049 Anti-Human CD4 PE-Cy7 (RPA-T4)
51-4732 Mouse IgG2b K Isotype Control Alexa Fluor® 647
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-0048 Anti-Human CD4 FITC 488 518 FC RUO
12-0048 Anti-Human CD4 PE 488 575 FC RUO
13-0048 Anti-Human CD4 Biotin N/A N/A FC RUO
14-0048 Anti-Human CD4 Purified N/A N/A FC IP RUO
16-0048 Anti-Human CD4 Functional Grade* Purified N/A N/A FC IP RUO
17-0048 Anti-Human CD4 APC 633 660 FC RUO
35-0048 Anti-Human CD4 PE-Cy5.5 (To Be Discontinued; See Replacement Formats: PerCP-eFluor® 710 or PerCP-Cy5.5) 488 690 FC RUO
45-0048 Anti-Human CD4 PerCP-Cy5.5 (PE-Cy5.5 replacement) 488 690 FC RUO
48-0048 Anti-Human CD4 eFluor® 450 (Pacific Blue® replacement) 405 450 FC RUO
51-0048 Anti-Human CD4 Alexa Fluor® 647 633 668 FC RUO
53-0048 Anti-Human CD4 Alexa Fluor® 488 488 519 FC RUO
56-0048 Anti-Human CD4 Alexa Fluor® 700 633 723 FC RUO
57-0048 DISCONTINUED - Refer to NEW Anti-Human CD4 eFluor® 450 (Pacific Blue® replacement) 402 421 FC RUO
93-0048 Anti-Human CD4 eFluor® 605NC 350-500 605 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.06 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)
Legal
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.