Staining of Molt-4 cells with Mouse IgG1 κ Isotype Control Alexa FluorŽ 647 (open histogram) or Anti-Human CD1b Alexa FluorŽ 647 (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Human CD1b Alexa FluorŽ 647 Catalog Number: 51-0018 Concentration: 0.2 mg/mL Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBioSN13 (SN13 K5-1B8) Host/Isotype: Mouse IgG1
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51-0018-71
25 tests
51-0018-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
Description
The eBioSN13 monoclonal antibody reacts with human CD1b. The CD1 molecules are similar to MHC class I, but rather than presenting peptide antigens to T-cell receptors (TCR), they present a diversity of lipid and glycolipid antigens which are derived from self as well as from mycobacteria which include sphingolipids, mycolic acid derivatives and phosphatidylinositols such as liparabinomannan (LAM) and glucose monomycolate (GMM). CD1b is 45 kDa, and like the other CD1 antigens, is expressed as a heterodimer composed of the CD1b noncovalently paired with β2-microglobulin.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioSN13 (SN13 K5-1B8) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioSN13 (SN13 K5-1B8) antibody has been pre-titrated and tested by flow cytometric analysis of the Molt-4 cell line. This can be used at 20 μl (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 ľL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C.
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9189-93.
Porcelli S, Morita CT, Brenner MB.
CD1b restricts the response of human CD4-8- T lymphocytes to a microbial antigen.
Nature. 1992 Dec 10;360(6404):593-7.
Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E.
Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells.
Infect Immun. 2001 Dec;69(12):7461-70. (SN13, FC, PubMed)
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
