Also known as: Ep-CAM, Epithelial cell adhesion molecule, KSA, TROP1
RUO: For Research Use Only
Staining of A549 cell line with Mouse IgG1 κ Isotype Control eFluorŪ 660 (cat. 50-4714) (blue histogram) or Anti-Human CD326 (EpCAM) eFluorŪ 660 (purple histogram). Total viable cells were used for analysis.
Contents: Anti-Human CD326 (EpCAM) eFluorŪ 660 (Alexa FluorŪ 647 replacement) Catalog Number: 50-9326 Concentration: 5 µl (0.03 µg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: 1B7 Host/Isotype: Mouse IgG1, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
50-9326-41
25 tests
50-9326-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 1B7 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of human A549 cell line. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
eFluorŪ 660 is a replacement for Alexa FluorŪ 647. eFluorŪ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
References
Suzuki K, Nakamura K, Kato K, Hamada H, Tsukamoto T. Exploration of target molecules for prostate cancer gene therapy. Prostate. 2007 Aug 1;67(11):1163-73. (1B7, FC, IHC, PubMed)
Rao CG, Chianese D, Doyle GV, Miller MC, Russell T, Sanders RA Jr, Terstappen LW. Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors. Int J Oncol. 2005 Jul;27(1):49-57. (PubMed)
Litvinov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO. Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2(5):417-28. (PubMed)
Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO. Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125(2):437-46. (PubMed)
